Second‐generation sequencing of entire mitochondrial coding‐regions (∼15.4 kb) holds promise for study of the phylogeny and taxonomy of human body lice and head lice
Identifieur interne : 000048 ( Istex/Checkpoint ); précédent : 000047; suivant : 000049Second‐generation sequencing of entire mitochondrial coding‐regions (∼15.4 kb) holds promise for study of the phylogeny and taxonomy of human body lice and head lice
Auteurs : H. Xiong [Australie] ; D. Campelo [Australie] ; R. J. Pollack [États-Unis] ; D. Raoult [France] ; R. Shao [Australie] ; M. Alem [Éthiopie] ; J. Ali [Éthiopie] ; K. Bilcha [Éthiopie] ; S. C. Barker [Australie]Source :
- Medical and Veterinary Entomology [ 0269-283X ] ; 2014-08.
Abstract
The Illumina Hiseq platform was used to sequence the entire mitochondrial coding‐regions of 20 body lice, Pediculus humanus Linnaeus, and head lice, P. capitis De Geer (Phthiraptera: Pediculidae), from eight towns and cities in five countries: Ethiopia, France, China, Australia and the U.S.A. These data (∼310 kb) were used to see how much more informative entire mitochondrial coding‐region sequences were than partial mitochondrial coding‐region sequences, and thus to guide the design of future studies of the phylogeny, origin, evolution and taxonomy of body lice and head lice. Phylogenies were compared from entire coding‐region sequences (∼15.4 kb), entire cox1 (∼1.5 kb), partial cox1 (∼700 bp) and partial cytb (∼600 bp) sequences. On the one hand, phylogenies from entire mitochondrial coding‐region sequences (∼15.4 kb) were much more informative than phylogenies from entire cox1 sequences (∼1.5 kb) and partial gene sequences (∼600 to ∼700 bp). For example, 19 branches had > 95% bootstrap support in our maximum likelihood tree from the entire mitochondrial coding‐regions (∼15.4 kb) whereas the tree from 700 bp cox1 had only two branches with bootstrap support > 95%. Yet, by contrast, partial cytb (∼600 bp) and partial cox1 (∼486 bp) sequences were sufficient to genotype lice to Clade A, B or C. The sequences of the mitochondrial genomes of the P. humanus, P. capitis and P. schaeffi Fahrenholz studied are in NCBI GenBank under the accession numbers KC660761‐800, KC685631‐6330, KC241882‐97, EU219988‐95, HM241895‐8 and JX080388‐407.
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DOI: 10.1111/mve.12076
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Ali</name><affiliation wicri:level="1"><country xml:lang="fr">Éthiopie</country><wicri:regionArea>Department of Microbiology, Immunology and Parasitology, College of Medicine and Health Sciences, University of Gondar, Gondar</wicri:regionArea><wicri:noRegion>Gondar</wicri:noRegion></affiliation></author><author><name sortKey="Bilcha, K" sort="Bilcha, K" uniqKey="Bilcha K" first="K." last="Bilcha">K. Bilcha</name><affiliation wicri:level="1"><country xml:lang="fr">Éthiopie</country><wicri:regionArea>Department of Dermatology and Venereology, College of Medicine and Health Sciences, University of Gondar, Gondar</wicri:regionArea><wicri:noRegion>Gondar</wicri:noRegion></affiliation></author><author><name sortKey="Barker, S C" sort="Barker, S C" uniqKey="Barker S" first="S. C." last="Barker">S. C. 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These data (∼310 kb) were used to see how much more informative entire mitochondrial coding‐region sequences were than partial mitochondrial coding‐region sequences, and thus to guide the design of future studies of the phylogeny, origin, evolution and taxonomy of body lice and head lice. Phylogenies were compared from entire coding‐region sequences (∼15.4 kb), entire cox1 (∼1.5 kb), partial cox1 (∼700 bp) and partial cytb (∼600 bp) sequences. On the one hand, phylogenies from entire mitochondrial coding‐region sequences (∼15.4 kb) were much more informative than phylogenies from entire cox1 sequences (∼1.5 kb) and partial gene sequences (∼600 to ∼700 bp). For example, 19 branches had > 95% bootstrap support in our maximum likelihood tree from the entire mitochondrial coding‐regions (∼15.4 kb) whereas the tree from 700 bp cox1 had only two branches with bootstrap support > 95%. Yet, by contrast, partial cytb (∼600 bp) and partial cox1 (∼486 bp) sequences were sufficient to genotype lice to Clade A, B or C. The sequences of the mitochondrial genomes of the P. humanus, P. capitis and P. schaeffi Fahrenholz studied are in NCBI GenBank under the accession numbers KC660761‐800, KC685631‐6330, KC241882‐97, EU219988‐95, HM241895‐8 and JX080388‐407.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>États-Unis</li><li>Éthiopie</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="Australie"><noRegion><name sortKey="Xiong, H" sort="Xiong, H" uniqKey="Xiong H" first="H." last="Xiong">H. Xiong</name></noRegion><name sortKey="Barker, S C" sort="Barker, S C" uniqKey="Barker S" first="S. C." last="Barker">S. C. Barker</name><name sortKey="Barker, S C" sort="Barker, S C" uniqKey="Barker S" first="S. C." last="Barker">S. C. 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